Genetic biochemistry of postlarval and juvenile stages of lobster Panulirus argus ( Latreille ). I - Heterozygocity related to metabolic activity, morphológical variation and environmental factors.

Authors

  • Raimundo Nonato de Lima Conceição Laboratório de Ciências do Mar, Universidade Federal do Ceará, C. P. 1072, CEP 60.165-080, Fortaleza, Brasil.
  • Georgina Espinosa López Laboratório de Ciências do Mar, Universidade Federal do Ceará, C. P. 1072, CEP 60.165-080, Fortaleza, Brasil.
  • Eugenio Díaz lglesia Centro de lnvestigaciones Marinas, Universidad de La Habana, Av. 1ª 2808, Miramar, La Habana, Cuba.
  • Marisabel Báez Hidalgo Centro de lnvestigaciones Marinas, Universidad de La Habana, Av. 1ª 2808, Miramar, La Habana, Cuba.
  • Rogélio Díaz Fernández Centro de lnvestigaciones Marinas, Universidad de La Habana, Av. 1ª 2808, Miramar, La Habana, Cuba.

DOI:

https://doi.org/10.32360/acmar.v29i1-2.31408

Keywords:

Lobster Panulirus argus, electrophoresis, genetic biochemistry.

Abstract

Electrophoresis is a process from which one can detec proteins variations and is based on the migration of genetic informations carrying molecules. In the present paper five proteins systems were studied and the heterogozygosity (H o) parameter, which represents genetic intra and interespecific variations along different life stages,was determined. The method used in the analysis was the vertical run in acrilamida gel. Samples were taken from the abdominal muscle of post-larvae and juveniles of lobster Panulirus argus. The systems studied were the esterases (EST); ma/ato deshidrogenase (MDH); glumatato
deshidrogenase ( GD H); tetrasoliun oxidase (TO) and total proteins (PT). The results of mean Ho were similar in puerulus, post-puerulus and juvenil stages. Comparing the central metabolism enzimes (group I) with the enzimes functionnig in the peripheric metabolism ways (group 11), it was observed that Ho for puerulus and post-puerulus stages were greater in group I enzimes than in group li, whereas in juveniles stages the reverse occurs. There was no Ho difference (P>O.OS) between males and females. A trend in the increase of Ho along ontogenic development was observed.

Published

2018-03-07

Issue

Section

Artigos originais