Lesões cerebrais e perfil hematoimunológico em Oreochromis niloticus após ingestão de ração infectada com Streptococcus agalactiae
DOI:
https://doi.org/10.5935/rbhsa.v16i4.691Palavras-chave:
Bacteriose, Tilápia, Infecção, Estreptococose.Resumo
O objetivo do estudo foi avaliar o tecido cerebral e o sangue de tilápia-do-nilo após ingestão de ração contaminada por Streptococcus agalactiae. 90 peixes foram alocados em seis unidades experimentais, sendo quatro tratamentos e duas unidades controle, totalizando 15 animais por tanque. Os peixes receberam o inóculo bacteriano via ração infectada, com um volume de 100 μL g ração –1 [109 UFC], enquanto os peixes do grupo controle receberam a mesma ração, sem o inóculo bacteriano. Foram realizadas duas coletas de amostras biológicas. A primeira coleta ocorreu no sétimo dia pós-infecção e a segunda coleta no décimo quarto dia pós-infecção. Amostras de tecido do cérebro e alíquotas de sangue foram coletadas de 24 peixes por tratamento para análises histológicas e hematoimunológicas. As análises histológicas do presente estudo revelaram que no tecido cerebral a intensidade das lesões não se altera entre o sétimo e o décimo quarto dia. Na hematoimunologia, houve alterações significativas entre o sétimo e o décimo quarto dia na CHCM, no hematócrito, no número de trombócitos e neutrófilos, bem como na quantidade de lisozima.
Referências
AGAMY, E. (2012). Histopathological liver alterations in juvenile rabbit fish (Siganus canaliculatus) exposed to light Arabian crude oil, dispersed oil and dispersant. Ecotoxicology and environmental safety, 75, 171-179. https://doi.org/10.1016/j.ecoenv.2011.09.010.
AMAL, M.N.A. & ZAMRI-SAAD, M. (2011). Streptococcosis in tilapia (Oreochromis niloticus): a review. Pertanika Journal of Tropical Agricultural Science, 34(2), 195-206.
AMAR, E.C.; KIRON, V.; SATOH, S.; OKAMOTO, N. & WATANABE, T. (2000). Effects of dietary β‐carotene on the immune response of rainbow trout Oncorhynchus mykiss. Fisheries Science, 66(6), 1068-1075. https://doi.org/10.1046/j.1444-2906.2000.00170.x.
BRUM, A.; CARDOSO, L.; CHAGAS, E.C.; CHAVES, F.C.M., MOURIÑO, J.L.P., & MARTINS, M.L. (2018). Histological changes in Nile tilapia fed essential oils of clove basil and ginger after challenge with Streptococcus agalactiae. Aquaculture, 490, 98-107. https://doi.org/10.1016/j.aquaculture.2018.02.040.
COLLIER, H.B. (1944). Standardization of blood haemoglobin determinations. Canadian Medical Association Journal, 50(6), 550-552.
FAZIO, F. (2019). Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture, 500, 237-242. https://doi.org/10.1016/j.aquaculture.2018.10.030.
GONG, M. (2014). Efficacy of lysozyme as an alternative to antibiotics for broiler chickens.
ISHIKAWA, N.M., RANZANI-PAIVA, M.J.T., LOMBARDI, J.V. (2008). Metodologia para quantificação de leucócitos totais em peixe, Oreochromis niloticus. Archives of Veterinary Science, 13, 54-63. http://hdl.handle.net/11449/70526.
JESUS, G.F.A.; OWATARI, M.S.; PEREIRA, S.A.; SILVA, B.C.; SYRACUSE, N.M.; LOPES, G.R.; ADDAM, K.; CARDOSO, L., MOURIÑO, J.L.P., & MARTINS, M.L. (2021). Effects of sodium butyrate and Lippia origanoides essential oil blend on growth, intestinal microbiota, histology, and haemato-immunological response of Nile tilapia. Fish & Shellfish Immunology, 117, 62-69. https://doi.org/10.1016/j.fsi.2021.07.008.
PAL, R.R.; BAIDYA, A.K.; MAMOU, G.; BHATTACHARYA, S.; SOCOL, Y.; KOBI, S.; KATSOWICH, N.; BEN-YEHUDA, S.; & ROSENSHINE, I. (2019). Pathogenic E. coli extracts nutrients from infected host cells utilizing injectisome components. Cell, 177(3), 683-696. https://doi.org/10.1016/j.cell.2019.02.022.
PALANG, I.; WITHYACHUMNARNKUL, B.; SENAPIN, S.; SIRIMANAPONG, W. & VANICHVIRIYAKIT, R. (2020). Brain histopathology in red tilapia Oreochromis sp. experimentally infected with Streptococcus agalactiae serotype III. Microscopy Research and Technique, 83(8), 877-888. https://doi.org/10.1002/jemt.23481.
POPMA, T.J. & LOVSHIN, L.L. (1996). Worldwide prospects for commercial production of tilapia. Alabama: International Center for Aquaculture and Aquatic Environments, 26 p.
PRIDGEON, J.W. & KLESIUS, P.H. (2012). Major bacterial diseases in aquaculture and their vaccine development. CABI Reviews, 7, 1-16. https://doi.org/10.1079/PAVSNNR20127048.
RANZANI-PAIVA, M.J.T.; DE PÁDUA, S.B., TAVARES-DIAS, M., & EGAMI, M.I. (2013). Métodos para análise hematológica em peixes. Editora da Universidade Estadual de Maringá-EDUEM.
RIBEIRO, H.J.; PROCÓPIO, M.S.; GOMES, J.M.M., VIEIRA, F.O.; RUSSO, R.C.; BALZUWEIT, K.; CHIARINI-GARCIA, H.; CASTRO, A.C. S.; RIZZO, C. & CORRÊA, J.D. (2011). Functional dissimilarity of melanomacrophage centres in the liver and spleen from females of the teleost fish Prochilodus argenteus. Cell and Tissue Research, 346(3), 417-425.
ROBERTS, R.J. (2012). Fish pathology. John Wiley & Sons. https://doi.org/10.1007/s00441-011-1286-3.
SAHOO, N.R.; KUMAR, P.; BHUSAN, B.; BHATTACHARYA, T.K.; DAYAL, S. & SAHOO, M. (2012). Lysozyme in livestock: a guide to selection for disease resistance: a review. Journal of Animal Science Advances, 2(4), 347-360.
SCHWAIGER, J.; WANKE, R.; ADAM, S.; PAWERT, M.; HONNEN, W. & TRIEBSKORN, R. (1997). The use of histopathological indicators to evaluate contaminant-related stress in fish. Journal of Aquatic Ecosystem Stress and Recovery, 6(1), 75-86. https://doi.org/10.1023/A:1008212000208.
SILVA, B.C.; MARTINS, M.L.; JATOBÁ, A.; BUGLIONE NETO, C.C.; VIEIRA, F.N.; PEREIRA, G.V.; JERÔNIMO, G.T.; SEIFFERT, W. Q. & MOURIÑO, J.L.P. (2009). Hematological and immunological responses of Nile tilapia after polyvalent vaccine administration by different routes. Pesquisa Veterinária Brasileira 29(11), 874-880. http://dx.doi.org/10.1590/S0100-736X2009001100002.
SOTO, E.; ZAYAS, M.; TOBAR, J.; ILLANES, O.; YOUNT, S.; FRANCIS, S. & DENNIS, M.M. (2016). Laboratory-controlled challenges of Nile tilapia (Oreochromis niloticus) with Streptococcus agalactiae: comparisons between immersion, oral, intracoelomic and intramuscular routes of infection. Journal of Comparative Pathology, 155 (4), 339-345. https://doi.org/10.1016/j.jcpa.2016.09.003.
SUANYUK, N.; KONG, F.; KO, D.; GILBERT, G.L.; SUPAMATTAYA, K.; 2008. Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia Oreochromis sp. and Nile tilapia O. niloticus in Thailand—relationship to human isolates? Aquaculture, 284 (1–4), 35–40. https://doi.org/10.1016/j.aquaculture.2008.07.034.
SUN, J.; FANG, W.; KE, B.; HE, D.; LIANG, Y.; NING, D.; TAN, H.; PENG, H.; WANG, Y.; MA, Y.; KE, C. & DENG X. (2016). Inapparent Streptococcus agalactiae infection in adult/commercial tilapia. Scientific Reports, 6, 26319. https://doi.org/10.1038/srep26319.
TATTIYAPONG, P.; DACHAVICHITLEAD, W. & SURACHETPONG, W. (2017). Experimental infection of Tilapia Lake Virus (TiLV) in Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis spp.). Veterinary Microbiology, 207, 170-177. https://doi.org/10.1016/j.vetmic.2017.06.014.
TAVARES-DIAS, M. & MARTINS, M. L. (2017). An overall estimation of losses caused by diseases in the Brazilian fish farms. Journal of Parasitic Diseases, 41(4), 913-918. https://doi.org/10.1007/s12639-017-0938-y.
THRALL, M.A. (2007). Hematologia e bioquímica clínica veterinária. Editora Roca.
TWETEN, R.K. & CAPARON, M. (2005). Injectosomes in Gram‐Positive Bacteria. Structural Biology of Bacterial Pathogenesis, 223-239. https://doi.org/10.1128/9781555818395.ch11.
YANONG, R. P., & FRANCIS-FLOYD, R. (2002). Streptococcal infections of fish. Florida Cooperative Extension Service. IFAS, University of Florida, pp. 1-5.
ZHANG, Z.; LAN, J.; LI, Y.; HU, M.; YU, A.; ZHANG, J., & WEI, S. (2018). The pathogenic and antimicrobial characteristics of an emerging Streptococcus agalactiae serotype IX in Tilapia. Microbial Pathogenesis, 122, 39-45. https://doi.org/10.1016/j.micpath.2018.05.053.
This is an open-access article distributed under the terms of the Creative Commons Attribution License
