FIRST EVALUATION OF GENOTOXICITY OF MACAÉ (RJ) ESTUARINE WATERS

Authors

  • Moisés Basilio da Conceição Laboratório de Biologia Molecular - Setor Genotoxicidade, NUPEM, UFRJ, Macaé, RJ, Brazil. Faculdade Salesiana Maria Auxiliadora, Macaé, RJ.
  • Vitor Oliveira da Costa Laboratório de Biologia Molecular - Setor Genotoxicidade, NUPEM, UFRJ, Macaé, RJ, Brazil.
  • Maurício Mussi Molisani Laboratório de Química, NUPEM, UFRJ.
  • Laura Isabel Weber Laboratório de Biologia Molecular - Setor Genotoxicidade, NUPEM, UFRJ, Macaé, RJ, Brazil.

DOI:

https://doi.org/10.32360/acmar.v50i2.31145

Keywords:

Macaé River, Danio rerio, Comet Assay, Micronuclei Test, Brazil.

Abstract

Low levels of contamination were detected recently on Macaé estuarine waters showing effects in aquatic organisms at different levels. Nonetheless, genotoxicity was not yet evaluated for these waters. A preliminary study was performed using zebrafish Danio rerio by exposing this species to Macaé River estuarine waters. DNA and chromosome damage was evaluated by the comet assay, after 24h and 48h of exposure; and by the micronucleus test, after 72 h and seven days of exposure. The results showed DNA damage (% Tail DNA) 5.1-fold higher in fish exposed to estuarine waters than in controls after 48 h of exposure; and significantly higher number of micronuclei/thousand (10-12) in fish exposed to estuarine waters than in controls after three and seven days of exposure. The results of the present study, although preliminary, evidenced genotoxicity of the estuarine waters from the Macaé River, indicating that genotoxicity was at levels to induce DNA and chromosome damage in adult fish. 

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Published

2018-08-20

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Section

Artigos originais