PRELIMINARY STUDY ON CELLULOLYTIC ACTIVITY OF Neoteredo reynei (BARTSCH, 1920) (MOLLUSCA: BIVALVIA: TEREDINIDAE)

Autores/as

  • Ana Fontenele Urano Carvalho Profa. Associada do Departamento de Biologia da Universidade Federal do Ceará.
  • Davi Felipe Farias Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Daniel Amorin Oriá Fernandes Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Lady Clarissa Brito Rocha Bezerra Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Liana Rodrigues Queiroz Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Marina Gomes Viana Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Fernanda Araújo Paes Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Hortência de Sousa Barroso Aluno de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Paulo César Medeiros Mesquita Alunos de graduação em Ciências Biológicas da Universidade Federal do Ceará
  • Vânia Maria Maciel Melo Profa. Adjunta do Departamento de Biologia da Universidade Federal do Ceará.
  • Cristina de Almeida Rocha Barreira Profa. Adjunta do Instituto de Ciências do Mar (LABOMAR) da Universidade Federal do Ceará

DOI:

https://doi.org/10.32360/acmar.v43i2.5993

Palabras clave:

Neoteredo reynei, cellulase, symbionts, digestion, cellulolytic activity

Resumen

This work aimed to assess the presence of cellulolytic activity in the microbiote isolated from the digestive tract of Neoteredo reynei and also the presence of cellulolytic activity intrinsic to the animal. To investigate cellulolytic activity in the gastrointestinal microbiote of N. reynei , the mollusks were aseptically dissected and samples of both gastrointestinal epithelium and gastrointestinalcontent were collected and then inoculated in nutritive agar plates for isolation of bacterial colonies. In order to investigate the mollusk intrinsic cellulolytic activity, some specimens had their whole bodies arbitrarily cut into four regions (shell region, anterior cecum region, posterior cecum region and pallet region) which constituted four different samples. Other specimens had their gastrointestinal content collected to obtain the fifth sample (lumen). All samples were sterilized by treatment with the antimicrobials tetracycline and cetoconazoland then macerated and submitted to cold extraction with sodium phosphate buffer 0.1 M, pH 6.0 (1:1; w/v) for 4 h. Then, extracts were filtered and centrifuged. The supernatant was separated for the cellulolytic assays which were performed in agar cellulose plates by radial diffusion method. Four out of five microbial isolates showed cellulolytic activity. Likewise, all body sections and lumen sterile samples of N. reynei showed cellulolytic activity. Thus, both host microbiote and the mollusk itself seem to be involved in cellulose digestion.

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Publicado

2010-11-01

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