Métodos de avaliação da limpeza e desinfecção de superfícies em unidade de terapia intensiva pediátrica

Flávia Silva Moreira; Mariana Anjos Mota; Luan Aparecido Alexandre Elias; Viviane Perbeline Gonçalves; Daniel de Macedo Rocha; Alvaro Francisco Lopes de Sousa; Aires Garcia dos Santos Junior

doi:10.36517/2175-6783.20252696009

Authors

Flávia Silva Moreira Federal University of Mato Grosso do Sul https://orcid.org/0009-0009-1553-7568
Mariana Anjos Mota Federal University of Mato Grosso do Sul https://orcid.org/0009-0003-4153-9686
Luan Aparecido Alexandre Elias Federal University of Mato Grosso do Sul https://orcid.org/0009-0004-7386-6873
Viviane Perbeline Gonçalves Federal University of Mato Grosso do Sul https://orcid.org/0000-0002-3245-175X
Daniel de Macedo Rocha Federal University of Mato Grosso do Sul https://orcid.org/0000-0003-1709-2143
Alvaro Francisco Lopes de Sousa Federal University of Mato Grosso do Sul https://orcid.org/0000-0003-2710-2122
Aires Garcia dos Santos Junior Federal University of Mato Grosso do Sul https://orcid.org/0000-0002-5946-0197

DOI:

https://doi.org/10.36517/2175-6783.20252696009

Keywords:

Pediatric Nursing; Biological Contamination; Infection Control; Intensive Care Units, Pediatric.

Abstract

Objective: to analyze the correlation between methods for evaluating the cleaning and disinfection of surfaces in a pediatric intensive care unit. Methods: a single-group, three-phase, quasi-experimental study conducted in a public unit. The effectiveness of cleaning 120 surfaces was assessed by performing 960 measurements before and after the process, using adenosine triphosphate quantification, visual inspection, and aerobic bacterial colony counts. Results: there was a significant positive correlation between adenosine triphosphate and colony-forming units only in phase three for the caregiver chair (Spearman’s rho = 0.74; p-value = 0.038). For the other surfaces, across the three phases, no significant correlations were observed between the two methods before or after cleaning and disinfection, indicating a lack of consistent association in most situations. Conclusion: the methods have distinct sensitivity and specificity profiles, are not interchangeable, and are influenced by the nature of the unit, the type of surface, and the equipment and supplies used. Contributions to practice: combining bioluminescence and fluorescence with visual inspection may strengthen infection prevention protocols, support managerial decisions, improve audit quality, and enhance nursing performance for patient safety in critical areas.

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