Métodos de avaliação da limpeza e desinfecção de superfícies em unidade de terapia intensiva pediátrica

Autores/as

DOI:

https://doi.org/10.36517/2175-6783.20252696009

Palabras clave:

Intensive Care Units

Resumen

Objetivo: analisar a correlação entre métodos de avaliação da limpeza e desinfecção de superfícies em unidade de terapia intensiva pediátrica. Métodos: estudo quase-experimental de grupo único, trifásico, realizado em unidade pública. Avaliaram-se a eficácia da higienização de 120 superfícies, realizando 960 medições antes e depois do processo, utilizando a quantificação de trifosfato de adenosina, inspeção visual e contagem de colônias de bactérias aeróbias. Resultados: houve correlação positiva e significativa entre trifosfato de adenosina e unidades formadoras de colônias apenas na fase três, para a poltrona do acompanhante (coeficiente de Spearman = 0,74; valor de probabilidade = 0,038). Nas demais superfícies, nas três fases, não se observaram correlações significativas entre os dois métodos, antes ou depois da limpeza e desinfecção, indicando ausência de associação consistente na maior parte das situações. Conclusão: os métodos apresentam características próprias de sensibilidade e especificidade, não se substituem e são influenciados pela natureza da unidade, pelo tipo de superfície e pelos equipamentos e insumos empregados. Contribuições para a prática: combinar bioluminescência e fluorescência com inspeção visual pode fortalecer protocolos de prevenção de infecções, apoiar decisões gerenciais, qualificar auditorias e aprimorar a atuação de enfermagem na segurança do paciente em áreas críticas.

Descargas

Los datos de descarga aún no están disponibles.

Referencias

1. Li T, Zhang H, Luo H, Li K, Huang N, Yang S, You J. Development and optimization of disinfection protocol for negative pressure wards: a Delphi study. BMC Infect Dis. 20257;25(1):1242. doi: https://doi.org/10.1186/s12879-025-11659-y

2. Nadi ZB, Raisali F, Jafari N, Bayramzadeh S. The influence of physical environment on health care–associated infections: a literature review. Am J Infect Control. 2024;52(2):229-42. doi: https://doi.org/10.1016/j.ajic.2023.06.010

3. Verhougstraete M, Cooksey E, Walker JP, Wilson AM, Lewis MS, Yoder A, et al. Impact of terminal cleaning in rooms previously occupied by patients with healthcare-associated infections. PLoS One. 2024;19(7):e0305083. doi: https://dx.doi.org/10.1371/journal.pone.0305083

4. Boyce JM. Hand and environmental hygiene: respective roles for MRSA, multi-resistant Gram negatives, Clostridioides difficile, and Candida spp. Antimicrob Resist Infect Control. 2024;13(1):110. doi:https://dx.doi.org/10.1186/s13756-024-01461-x

5. Alqurashi MS, Sawan AA, Berekaa MM, Hunasemarada BC, Al Shubbar MD, Al Qunais AA, et al. Hospital hygiene paradox: MRSA and Enterobacteriaceae colonization among cleaning staff in a tertiary hospital in Saudi Arabia. Medicina (Kaunas). 2025;61(3):384. doi: http://dx.doi.org/10.3390/medicina61030384

6. Hong W, Nyaruaba R, Li X, Liu H, Yang H, Wei H. In-situ and real-time monitoring of the interaction between lysins and staphylococcus aureus biofilm by surface plasmon resonance. Front Microbiol. 2021;12:783472. doi: https://doi.org/10.3389/fmicb.2021.783472

7. Silva MHN, Ciofi-Silva CL, Souza VS, Rocha DM, Sousa AFL, Felix AMS, et al. Comparative effectiveness of a multimodal intervention package on surface cleaning and disinfection in Brazilian neonatal intensive care units. Front Public Health. 2025;13:1557538. doi: https://doi.org/10.3389/fpubh.2025.1557538

8. Leistner R, Kohlmorgen B, Brodzinski A, Schwab F, Lemke E, Zakonsky G, et al. Environmental cleaning to prevent hospital-acquired infections on non-intensive care units: a pragmatic cluster randomized trial. EClinicalMedicine. 2023;59:101958. doi: https://dx.doi.org/10.1016/j.eclinm.2023.101958

9. Rigonato EM, Lima HP, Sousa AFL, Santos JLG, Jorge BM, Klock P, et al. Cleaning and disinfection of surfaces in a COVID-19 respiratory syndrome unit: a quasi-experimental study. Coronaviruses. 2024;5(2):88-96. doi: https://doi.org/10.2174/0126667975333048241025050846

10. Artasensi A, Mazzotta S, Fumagalli L. Back to basics: choosing the appropriate surface disinfectant. Antibiotics (Basel). 2021;10(6):613. doi: https://doi.org/10.3390/antibiotics10060613

11. Tseng WC, Wang YC, Chen WC, Lin KP. Utilizing an adenosine triphosphate bioluminescence assay as an indicator of instrument and environmental cleanliness in the radiology department: a pilot study. Infect Prev Pract. 2025;7(2):100449. doi: https://doi.org/10.1016/j.infpip.2025.100449

12. Niephaus V, Parohl N, Heiligtag S, Reuter H, Hackler R, Popp W. Can the ATP bioluminescence assay be used as an indicator for hospital cleaning? GMS Hyg Infect Control. 2024;19:Doc07. doi: https://doi.org/10.3205/dgkh000462

13. Frota OP, Santos EA, Oliveira SM, Silva LR, Sousa AFL. Efficiency of surface cleaning and disinfection: correlation between evaluation methods. Rev Bras Enferm. 2017;70(6):1176-83. doi: https://doi.org/10.1590/0034-7167-2016-0608

14. Frota OP, Ferreira AM, Rigotti MA, Andrade D, Borges NMA, Ferreira Júnior MA. Effectiveness of clinical surface cleaning and disinfection: evaluation methods. Rev Bras Enferm. 2020;73(1):e20180623. doi: https://dx.doi.org/10.1590/0034-7167-2018-0623

15. Xiong J, Jiang W, Wan W, Zuo L, Tu P, Rao DX. Improving precision instrument cleaning with a quality control module: implementation and outcomes. Front Public Health. 2025;13:1553113. doi: https://doi.org/10.3389/fpubh.2025.1553113

16. Fieldhouse S, Bastaki BB, Ledgerton A, Clarke P, Lewis T. Assessing Hospital Cleaning Effectiveness Using Fluorescence: A Proof-of-Concept Study and Comparison to ATP Testing. J Hosp Infect. 2025;9:S0195-6701(25)00267-1. doi: http://doi.org/10.1016/j.jhin.2025.08.008

17. Chan ASF, Chan HLY, Yan BKL, Lai MKC. Effectiveness of adenosine triphosphate to monitor manual cleaning and disinfection efficacy of flexible endoscopes in Hong Kong. JGH Open. 2023;7(2):141-7. doi: https://doi.org/10.1002/jgh3.12863

18. Boyce JM, Havill NL, Dumigan DG, Golebiewski M, Balogun O, Rizvani R. Monitoring the effectiveness of hospital cleaning practices by use of an ATP bioluminescence assay. Infect Control Hosp Epidemiol. 2009;30(7):678-84. doi: https://doi.org/10.1086/598243

19. Wu Y, Li L. An investigation into cleaning quality of suction-type metal lumen instruments: a cross-sectional study. Sci Rep. 2025;15(1):2492. doi: https://doi.org/10.1038/s41598-024-83215-8

20. Chen YC, Huang HM, Lin PY, Shi ZY. Comparing visual inspection and performance observation for evaluation of hospital cleanliness. Am J Infect Control. 2021;49(12):1511-14. doi: https://doi.org/10.1016/j.ajic.2021.07.011

21. Weber DJ, Rutala WA, Anderson DJ, Sickbert-Bennett EE. Biofilms on medical instruments and surfaces: do they interfere with instrument reprocessing and surface disinfection? Am J Infect Control. 2023;51(11):114-9. doi: https://doi.org/10.1016/j.ajic.2023.04.158

22. Whiteley GS, Glasbey TO, Fahey PP. Using a simplified ATP algorithm to improve data reliability and improve cleanliness standards for surface and medical device hygiene. Infect Dis Health. 2022;27(1):3-9. doi: http://dx.doi.org/10.1016/j.idh.2021.07.001

23. Komamizu S, Yamamoto Y, Morikane K, Kuwabara Y, Kondo M, Tatebayashi K, et al. Clinical and microbiological effectiveness of pulsed-xenon ultraviolet light disinfection in a neonatal intensive care unit in Japan. J Hosp Infect. 2025;156:13-6. doi: https://doi.org/10.1016/j.jhin.2024.11.015

24. Ishikawa K, Tsuchida T, Ichiki K, Ueda T, Yamada K, Iijima K, et al. Efficacy of enhanced environmental cleaning/disinfection using pulsed xenon ultraviolet light in preventing MRSA outbreaks in neonatal intensive care units. Epidemiologia. 2025;6(1):12. doi: https://doi.org/10.3390/epidemiologia6010012

25. Dramowski A, Aucamp M, Bekker A, Pillay S, Moloto K, Whitelaw AC, Cotton MF, Coffin S. NeoCLEAN: a multimodal strategy to enhance environmental cleaning in a resource-limited neonatal unit. Antimicrob Resist Infect Control. 2021;10(1):35. doi: https://doi.org/10.1186/s13756-021-00905-y

26. van Arkel A, Willemsen I, Kluytmans J. The correlation between ATP measurement and microbial contamination of inanimate surfaces. Antimicrob Resist Infect Control. 2021;10(1):116. doi: https://doi.org/10.1186/s13756-021-00981-0

27. Salih AN, Allo RR. Evaluation of nursing intervention measures in infection control at dialysis units in Mosul city hospitals. Med J Babylon. 2024;21(2):245-50. doi: http://doi.org/10.4103/MJBL.MJBL_360_22

28. Gastaldi S, Accorgi D, D’Ancona F. Tools and strategies for monitoring hospital environmental hygiene services. J Hosp Infect. 2025;159:52-61. doi: https://doi.org/10.1016/j.jhin.2025.01.011

Publicado

2025-11-13

Número

Sección

Artigos de Pesquisa

Cómo citar

1.
Moreira FS, Mota MA, Elias LAA, Gonçalves VP, Rocha D de M, Sousa AFL de, et al. Métodos de avaliação da limpeza e desinfecção de superfícies em unidade de terapia intensiva pediátrica. Rev Rene [Internet]. 2025 Nov. 13 [cited 2026 Mar. 2];26:e96009. Available from: https://periodicos.ufc.br/rene/article/view/96009