Use of different substrate for inoculum production of Pleurotus ostreatoroseus Sing.
Authors
Eduardo Bernardi
Universidade Federal de Pelotas
Lorena Donini
Universidade Federal de Pelotas
Elisandra Minotto
Universidade Federal de Pelotas
José Nascimento
Universidade Federal de Pelotas
Keywords:
shimeji, mycelium growth, substrate, spawn
Abstract
The objective of this work was to evaluate the mycelium growth of Pleurotus ostreatoroseus (strain POR 01/03) in different substrata, trying to make possible the output of this mushroom. The substrata of black oats, white oats, peeled oats, ryegrass, sunflower, corn, soy, wheat, peel of rice, sawdust, rice in peel and impurity of rice were cooked and conditioned in test tubes (2,5 x 20 cm), being later sterilized at 121ºC for 30 minutes. Disks of mycelium with 10mm of diameter were pealed for the tubes, which were incubated at 28ºC until the complete colonization. The growth measurements were accomplished every 48 hours. Among the evaluated substrata, the black oats and the sawdust added with 20% of soy crumb were the most suitable for the production of inoculum of Pleurotus ostreatoroseus.