This work was carried out to determine a methodology for the in vitro multiplication of Spathiphyllum wallisi. The rhizome segments were disinfected using calcium hypochlorite and sodium hypochlorite at 2% with and without immersion in alcohol 70%. In the multiplication, the stem segments removed from multiplied plants and kept in vitro were inoculated in MS medium with different concentrations of BAP and ANA. For root induction, the explants were inoculated in different concentrations of the MS medium salts, and different concentrations of AIB. It was used for all the experiments a complete rondomized design with treatments disposed in factorial arrangement. The immersion in sodium hypochlorite was the best treatment for the explant combination desinfestation/survival. For the multiplication experiment the greatest buds emission was observed with 2.0 mg L-1 BAP, regardless of ANA presence. For the explants rooting the MS medium in its normal concentration (100%), with 1.0 mg L-1 AIB, enable rooting with great developed plant and well formed roots”.
