ENZIMATIC HYDROLYSIS OF THE RAW MUSCLE OF NURSE SHARK, Gynglimostoma cirratum

Authors

  • Maria Petronília de Oliveira Studart Gurgel Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici, Fortaleza
  • Gustavo H. Fernandes Vieira Departamento de Biologia, Universidade do Vale do Acaraú, Sobral, Estado do Ceará
  • Regine Helena Silva dos Fernandes Vieira Departamento de Biologia, Universidade do Vale do Acaraú, Sobral, Estado do Ceará
  • Antonio M. Martin Memorial University of Newfoundland, Canadá

DOI:

https://doi.org/10.32360/acmar.v35i1-2.30909

Keywords:

Gynglimostoma cirratum, hydrolysis, fish concentarte, protein.

Abstract

This paper reports studies on the hydrolysis of the raw muscle of nurse shark, Gynglimostoma cirratum. The material was washed three times with distilled water and diluted in water in a 1:2 muscle/water ratio. The pH was maintaned at 7.,5 and pancreatin, papain or protease were added in the proportions of 0.1%, 0,2%, 0,3%, 0,4% and 0,5% of the total weight of shark muscle. The hydrolysis took place in na agitated aqueous medium at 40°C. The environmental pH was measured at 5, 10, 15, 30, 60, 90 and 120 min. If required, 0,1% NaOH was added to keep the pH at 8.5. The enzymatic reaction rate was determined by the degree of hydrolysis. The statistical analysis showed there to be significant diferences in the results as a function of the enzymes, times and enzymatic concentrations used. The best results were produced with the enzyme pancreatin; otimal reaction times were found at 90 min. and 120 min., and optimal enzymatic concentrations were found at 0,4 and 0,5%. Cost factors suggest the use of pancreatin at 0.4%. By choosing a reaction time of 90 min. there will be less risks of bacterial contamination.

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Published

2017-12-14

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Section

Artigos originais