ENZIMATIC HYDROLYSIS OF THE RAW MUSCLE OF NURSE SHARK, Gynglimostoma cirratum

Abstract

This paper reports studies on the hydrolysis of the raw muscle of nurse shark, Gynglimostoma cirratum. The material was washed three times with distilled water and diluted in water in a 1:2 muscle/water ratio. The pH was maintaned at 7.,5 and pancreatin, papain or protease were added in the proportions of 0.1%, 0,2%, 0,3%, 0,4% and 0,5% of the total weight of shark muscle. The hydrolysis took place in na agitated aqueous medium at 40°C. The environmental pH was measured at 5, 10, 15, 30, 60, 90 and 120 min. If required, 0,1% NaOH was added to keep the pH at 8.5. The enzymatic reaction rate was determined by the degree of hydrolysis. The statistical analysis showed there to be significant diferences in the results as a function of the enzymes, times and enzymatic concentrations used. The best results were produced with the enzyme pancreatin; otimal reaction times were found at 90 min. and 120 min., and optimal enzymatic concentrations were found at 0,4 and 0,5%. Cost factors suggest the use of pancreatin at 0.4%. By choosing a reaction time of 90 min. there will be less risks of bacterial contamination.